Specifications for dotSlide |
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 Archiving rare and valuable
biological sections
Sharing specimen via the web for
discussion, presentation or
teleconferencing
Building online databases for
students education
Setting up application-oriented
reference databases |
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Fully automatic virtual slide scanner
- dotSlide BX microscope frame
- Objectives included: 2x PLAPON, 10x, 20x, 40x UPLSAPO, other objectives may be added optionally, e.g. 100x oil
- Illumination: brightfield with 100 W halogen lamp house, optional: fluorescence, polarisation
- Slide holder for 1” x 3” slides and for 2” x 3” slides
- Multistage insert for batch scanning of four 1’’x 3’’ slides (optional)
- Slide loader for 50 slides 3’ x 1’, with integrated bar code scanner (optional)
- Digital CCD camera: 2/3” CCD camera, 6.45 µm x 6.45 µm pixel size, high sensitivity, high resolution, Peltier cooled, dynamic range of 3 x 12 bit
- dotSlide workstation with Dual XEON™ technology and 500 GB data storage capacity, network interface 100/1,000 Mbit/s Ethernet 4 GB RAM and high-resolution TFT monitor
Data storage solution (optional)
- Dimensions and weight (H x W x D), excluding workstation and monitor: system w/o slide loader: 50 cm x 45 cm x 48 cm, 30 kg; system incl. slide loader: 50 cm x 112 cm x 48 cm, 80 kg
- CE compliance
Resolution
- High-resolution scanning (standard 1x camera adapter): 0.32 µm/pixel (20x/NA 0.75) and 0.16 µm/pixel (40x/NA 0.9)
- Fast scanning (0.63x camera adapter option): 0.51µm/pixel (20x/NA 0.75) and 0.26 µm/pixel (40x/NA 0.9)
Scanning speed
- 10 x 10mm sample area with 20x objective:
- High-resolution scanning < 3 min
- Optional: fast scanning mode < 2 min
Image acquisition
- Automatic tissue finding
- Automatic image montage
- Seamless stitching
- Single button for automatic scanning of up to 50 slides (with optional slide loader)
- Focus points are taken automatically on the sample area. Different focus map algorithms are selectable for an optimal result with different types of samples.
- Data compression takes place during the scanning process without any time delay. Algorithms: JPEG, lossless JPEG, JPEG 2000 or no compression.
Software functions and modules
- View images locally, zoom, crop and rotate images
- Compress images and save images in different file formats
- Insert text annotations and drawings; create views of regions of interest, record audio annotations (sound card, microphone and speakers required)
- Net Image Server software for remote access to virtual slides with dotSlide software, OlyVIA viewer or Web browser, management of virtual slides and user access to images
- Conferencing module for simultaneous and synchronised viewing of virtual slides, conference access control and user management, minute taking tool, chat function
- TMA module to acquire individual images of TMA cores
Analysis
- Integrated measurement functions: distances, areas, circumferences, point count, export results to Microsoft Excel
- Analysis with cell* software packages, e.g. phase analysis to measure shares of tissue types, particle analysis to identify content of tumour cells
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