Specifications for CellCut Plus

Ultra precise and versatile system
Outstanding optical quality
Provides high quality starting material
    for sensitive downstream analysis 

Intuitive and powerful UVCut software
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Key FeaturesSpecificationsApplications

Frames
Microdissection Components
Observation Tubes, Intermediate Tubes and Eyepieces
Illumination Pillar, Lamphouse and Power Supply
Condenser
Range of Objectives
Fluorescence Equipment (optional)
Motorisation (with IX81)


Frames

Name Description Article code
IX71S8F-3 Manual inverted frame with left-side tilted sextuple nosepiece, field number 22 and light-path prism to left-side image port (80%). For transmitted and reflected illumination. Coarse and fine focussing knobs with maximum focus stroke of 9 mm and 100 µm stroke per full rotation of the fine focus knob. Minimum fine graduation 1 µm. Front control of the external light power supply. With integrated magnification changer 1.0x and 1.6x. Prepared for control of external devices via TTL pulse control switch. Cable to connect frame and external Olympus light power supply included..  N1509400
 IX81S8F Motorised inverted frame with left-side tilted motorised sextuple nosepiece, field number 22 and motorised light-path prisum to left-side image port (80%).  For transmitted and reflected illumination.  Motorised internal Z-drive with 0.01µm
 minimum step size. Coarse and fine focussing knobs with maximum focus stroke of 9 mm. Minimum fine graduation 0.1 µm. Programmable frame keys for Z-focus escape/return, coarse/fine switching, changing of the lightpath prism and light intensity control. Indication of light intensity via front LED display. External control of microscope functions via handswitch and/or PC (RS232 communication). Cables to connect frame and IX2 controlbox included.
 E0432000
 E0432000
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Microdissection Components

Name  Description Article Code
Laser

Solid state UV-A laser  for cutting and isolation of target samples, 355 nm, pulse duration smaller 500psec, with repetitionrate bigger than 5KHz,
maintenance free,  guaranteed lifespan 5000 hrs. or minimum 1 year, 10.000 hrs. expected
Fixed beam aligned in optical axis
Extremely sharp focus due to computer simulated optics
Computer controlled laser power
Computer controlled laser focus (z-position of the beam)
Safety devices and eye protection filters

Control Unit System control unit with complete set of wires.
Computer controlled xy-stage Computer controlled and motorised xy-stage 120x100 mm travelling path. 0,025 µm step width for high precision cutting. 1µm repositioning accuracy
CapLift Software controlled positioning, lifting and lowering of adhesive cap, possibility to adjust contact pressure according to specific application needs.
Camera

CellCamera DXA285cF for outstanding image quality and colour fidelity
IEEE 1394a (FireWireTM progressive scan CCD camera)
Ultra high sensivity Exview CCD technology and dynamic range
Advanced passive cooling concept for exposure times up to 30 sec
1392 x 1040 pixels
High Quality 12 Bit slow scan mode for lowest readout noise
Up to 15 full frames per second
Ultra compact dimensions
Automatic shutter, automatic/manual white balance

UV Cut Software
(system control software)
Special program for drawing the cutting path into the computer live image.
Free-hand drawing or predefined figures
Automatic cutting of marked areas or cells
Multigroup marking and cutting of unlimited number of different groups
Multiple cutting of the same area
Automatic Cap Inspection Mode and automatic CapLift over full slide
Store and recall of positions
Documentation
Scanned-in overview of slide for easy navigation
Modular Software, compatible with Optical Tweezers and Cell Pick
Unique PTP (Predefined Target Positioning) functions
System PC and monitor System computer and monitor
Interactive control of motorised xy stage with real time video image on computer screen.
Complete with computer Pentium IV 650, 3.4 GHz, 1 GB RAM, MS Windows XP Professional, HDD 80 GB, video overlay, 19” LCD flat screen,  DVD burner, RW
CellCut Documentation Complete instruction manual in English as hardcopy and as .doc file on system computer
Consumables 50 membrane slides, 50 caps with adhesive material
E0439103
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Observation Tubes, Intermediate Tubes and Eyepieces

Description Article code
Observation Tubes
U-BI90CT-1-2 Binocular (Siedentopf) tube with intergrated centring telescope and shutter, 45° inclination and pupillary distance adjustment from 50 to 76 mm. Adjustable diopter range (± 5 diopter) on right sleeve. To be used with WHN10x/22, WHN10x-H/22 eye-pieces. N1510300
U-TBI90 Binocular ergo tube with 35° to 85° tilting angle, diopter adjustment on one eyepiece, eyepoint height adjustable from 406mm to 471 mm. 035774
Intermediate Tubes
U-TV0.63XC Camera adapter with C-mount, 0.63x magnification factor. N2179000
Eyepieces
WHN10X/22 Widefield eye-piece with fieldnumber 22. N1508000
WHN10X-H/22 Focusable widefield eye-piece with fieldnumber 22. N1508100
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Illumination Pillar, Lamphouse and Power Supply

Description Article code
Illumination pillar (100W) 
IX2-ILL100 Tiltable illumination pillar for centerable 100 W Halogen lamp housing, 4 filter holders, F.S. and condenser support. 35762
Lamphouse and power supply (100W) 
U-LH100L-3-5 100 W Halogen lamphouse with collector lens and long connecting cable. N2212100 
 U-RMT  Cable for connecting the 100 W Halogen lamp house U-LH100-3-5 or IX-HLS100 with TH-4 transformer or the BX- or IX2-UCB control-boxes for PC control of the lamp.  Length 1.7m  37998

           

AHS-LAMP 12V/100W

Precentered 100 W halogen lamp

35359

TH-4 Transformer for 100 W Halogen lamp, 220-240 V. 35495
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Condenser

Description Article code
IX-MLWCD Universal ultra long working distance condenser for brightfield, Olympus relief contrast, phase contrast and DIC. With numerical aperture of 0.5, working distance of 45 mm. Equipped with aperture stop and polariser for relief contrast. Four-position turret includes the relief contrast elements RC1, RC2 and RC3. 35771
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Range of Objectives

Description Article Code
Note: Only the following objectives are offered for cutting. The full range of objectives can be found under Objectives & Accessories. For the optimal choice of objectives according to your application please Contact Us.
UPLFLN4x/0,13 Universal Plan Fluorite objective with 4x magnification. With working distance of 17 mm, and numerical aperture of 0.13. Suitable for any cover slip thickness. N1478300
UPLFLN10x/0,3 Universal Plan Fluorite objective with 10x magnification. With working distance of 10 mm und numerical aperture of 0.3. Suitable for any cover slip thickness. N1478400
UPLFLN10XPH/0,3 Universal Plan Fluorite objective with 10x magnification. With working distance of 10 mm and numerical aperture of 0.3. Suitable for any cover slip thickness. For phase contrast observations (PH1). N1479200
LUCPLFLN20X/0.45 Long working distance Universal CPlan Fluorite objective with 20x magnification. With variable working distance from 6.6 to 7.8 mm, numerical aperture of 0.45 and variable cover correction from 0 to 2 mm via correction collar. N1492700
LUCPLFLN20XPH/0.45 Long working distance Universal Cplan Fluorite objective with 20x magnification. With variable working distance from 6.6 to 7.8 mm, numerical aperture of 0.45 and variable cover correction from 0 to 2 mm via correction collar. For phase-contrast observations (PH1). N1492900
LUCPLFLN40X/0,6 Long working distance Universal CPlan Fluorite objective with 40x magnification. With variable working distance from 2.7 to 4 mm, numerical aperture of 0.6 and variable cover correction from 0 to 2 mm via focus free correction collar. N1492800
LUCPLFLN40XPH/0.6 Long working distance Universal Cplan Fluorite objective with 40x magnification. With variable working distance from 3 to 4.2 mm, numerical aperture of 0.6 and variable cover correction from 0 to 2 mm via focus free correction collar. For phase-contrast observations (PH2). N1493000
LUCPLFLN60X/0,7 Long working distance Universal CPlan Fluorite objective with 60x magnification. With variable working distance from 1.5 to 2.2 mm, numerical aperture of 0.7 and variable cover correction from 0.1 to 1.3 mm via semi focus free correction collar. N1507100
LUCPLFLN60XPH/0.7 Long working distance Universal CPlan Fluorite objective with 60x magnification. With variable working distance from 1.5 to 2.2 mm, numerical aperture of 0.7 and variable cover correction from 0.1 to 1.3 mm via semi focus free correction collar. For phase-contrast observations (PH2). N1507200
UPLFLN100xO2/1.3 Universal Plan Fluorite oil immersion objective with 100x magnification. With working distance of 0.2 mm, numerical aperture of 1.3 and cover correction of 0.17 mm. N1561200
UPLFLN100xO2PH/1.3 Universal Plan Fluorite oil immersion objective with 100x magnification. With working distance of 0.2 mm, numerical aperture of 1.3 and cover correction of 0.17 mm. For phase-contrast observations (PH3). N1561400
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Fluorescence Equipment (optional)

Description Article Code
Fluorescence Adaption
MMI-FLUO-AD Integration of Olympus fluorescence items plus SW functions E0439239
Lamphouse, Powersupply and Burner
U-LH100HG Lamphouse incl. lamp socket and aspherical collector lens for 100W mercury lamp. 037940
U-RFL-T2-200 Power supply for 100W mercury lamp, 220-240 V/50-60Hz, 3 fuses for replacement. 037947
USH-103OL USHIO 100W mercury burner N1217800
UYCP Power cord 037940
Fluorescence Condenser
IX2-RFAL L-shaped fluorescence condenser, with centerable aperture stop, field stop and filter slider 35764
Fluorescence Cubes
U-MNU2 Fluorescence filter cube, narrow-band UV excitation: exciter filter BP360-370, beam splitter DM400, barrier filter BA420 37056
U-MWIBA3 Fluorescence filter cube with Ion-coated filters. Wide-band Blueexcitation with bandpass emitter: exciter filter BP460-495, dichroic beam splitter DM505, barrier BA510-550 N1511000
U-MWG2 Fluorescence filter cube, wide-band Green excitation: exciter filter BP510-550, dichroic beam splitter DM570, barrier filter BA590 37063
IX2-RFAC Fluorescence cube cassette, six-position turret and manual shutter. 35765
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Motorisation (with IX81)

Name Description Article Code
IX2-UCB-2 Control box for the motorised units, equipped with power source for U-LH100L-3, including IX2-BSW basis software N1169600
U-ZPCB Z-drive control board for the UCB control units, necessary for operating the motorised focusing unit for the motorised frames of the BX2 and IX2 series 37996
UYCP Power Cord 35425
BX-UCBRS232 Special RS232 cable (with socket/socket) for connecting the control box with the PC. 39638 
Motorised Fluorescence Components
IX2-RFAL L-shaped fluorescence condenser, with centerable aperture stop, field stop and filter slider 35764
IX2-RFACA Motorised six-position fluorescence cube cassette with manual shutter 35766
IX2-SHA Motorised shutter for reflected and transmitted light 35801
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