Key Features for FV1000 |
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 Unmatched 2nm wavelength resolution
Ideal for time resolved spectroscopy
Highest sensitivity for minimal cell
damage
Powerful software, easy to handle |
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Simultaneous Laser Light Stimulation and Imaging (SIM Scanner)
The FV1000 incorporates 2 laser scanners in a single compact design for simultaneous confocal fluorescence observation and independent laser light stimulation. Synchronization of these two functions ensures that rapid cellular reactions that occur during or immediately following stimulation are not overlooked. Any region on interest can be specified for stimulation and scanning independently, with unrestricted control of variations in timing, duration and intensity. The circular “Tornado” scan provides highly efficient photobleaching and photoactivation in contrast to standard raster-scan patterns.
The SIM scanner makes the FV1000 the most suitable confocal microscope for a variety of applications, including FRAP, FLIP, photoactivation, photoconversion, uncaging, laser ablation and many others. |
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Spectral Detection System for accurate separation of overlapping emission spectrums
The original spectral detection system features two independent spectral detection channels, each configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection. Accurate spectral unmixing of overlapping fluorescence emissions becomes possible with a linear resolution of 2nm throughout the visible spectrum (400 to 800nm). Two different spectral deconvolution algorithms, Normal and Blind deconvolution, allow an easy and fast separation of different but overlapping fluochromes within the specimen.
High-speed spectroscopy can be performed with a maximum speed of 100nm/msec. |
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High Sensitivity in the fluorescence detection – ideal for live cell observation
The new optical concept and the high-sensitivity detection system allow efficient fluorescence detection, which minimizes the damage of living cells.
All FV1000 filters feature an ion sputter coating applied by ion deposition technology. This original Olympus technology provides filters with superior curve steepness and wavelength band transmission compared to conventional vacuum deposition. The resulting extended emission bandwidths and increased transmission efficiency enable observation with reduced excitation laser intensity, minimizing photo damage to living cells.
High-sensitivity PMTs, selected for high efficiency, can be used in either an Analog Accumulation (AAC) mode or in a Hybrid photon Counting Mode (HPCM), ideal for low light specimens. |
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High Precision and High Speed for accurate and reliable experiment analysis
The unique laser feedback system of the FV1000 provides stable laser excitation throughout the time course of living cell studies; a necessary feature for accurate fluorescence quantification. Furthermore the FV1000 employs an external real time controller for high precision time-lapse imaging of living cells. Data acquisition is controlled in microsecond increments, ensuring far more accurate and reliable timing.
The incorporation of highly stable galvano-meter scanning mirrors and an Olympus original acceleration circuit enables high-speed imaging (16 frames per second at 256x256 image format). Observation of rapid changes with living cells is now possible with significantly increased time resolution. |
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High modular system design realises configurations adapted to researchers needs
Due to the new optical concept of the Olympus FV1000 any motorised Olympus microscope frame (BX61, BX61WI and IX81) can be upgraded with the FV1000 to set up a high-end confocal system.
The modular design of the system makes a configuration based on individual researchers needs possible. |
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User friendly application Software – ideal for Multi-user environments
The graphical user interface of the FV1000 is designed for easy and quick operation of the complex system without sacrificing the possibility of high end experiment analyses, such as co-localization and ratio imaging. This straight on design makes the FV1000 ideal for multi user environments.
The implemented protocol processor enables the user to compute complex experiments by drag and drop in a convincing fast and easy way.
The acquired images can be stored in a wide range of file formats for flexible data. |
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Unique focus shift corrected LSM objectives
Olympus offers special focus shift corrected objectives for the use with the confocal FluoView systems. Those objectives are corrected from 400 to 750nm and show no depth shift between e.g. blue and red fluorescence, making them indispensable for e.g. co-localization analyses. |
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FV1000 related Application Report |
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