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Stable and clear system design The Olympus FluoView 300 confocal systems are fully integrated workstation that incorporate userfriendly image acquisition and image analysis with high-resolution confocal optics that require no user alignment. |
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Simultaneous acquisition of up to three channels The optical principle of the FluoView features the simultaneous acquisition of up to three channels with one scan. Beside of the two confocal fluorescence channels a transmitted light image (Brightfield or DIC) can be recorded. This transmitted light image is giving extremely helpful information if overlaid by the confocal fluorescence image. |
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A broad range of lasers are available to suit a maximum number of biological applications A wide range of lasers can be adapted to the FV300 system. Excitation wavelengths like 457, 488, 515, 543 and 633nm can be used to excite a broad range of known fluochromes. In addition the 440nm laser diode is available for CFP/YFP FRET applications, providing optimal excitation of CFP and spectral separation from the YFP signal. Each laser wavelength may be independently regulated thorough software-controlled neutral density filter wheels of through an acousto-optical filter (AOTF) for simultaneous or automated-sequential collection of multi-channel images. |
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405nm laser diode with unique laser modulation For most IV imaging applications, including nuclei imaging with DAPI, the 405nm laser diode is available. The unique laser modulation of the 405nm diode permits high-speed control of light intensity, which can be important for photoactivation and photobleaching studies. The exceptional light stability of the 405nm laser diode is also important for time-lapse studies of living cells. |
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User friendly application Software – ideal for Multi-user environments The graphical user interface of the FV1000 is designed for easy and quick operation of the complex system without sacrificing the possibility of high end experiment analyses, such as co-localization and ratio imaging. This straight on design makes the FV1000 ideal for multi user environments. The implemented protocol processor enables the user to compute complex experiments by drag and drop in a convincing fast and easy way. The acquired images can be stored in a wide range of file formats for flexible data. |
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Unique focus shift corrected LSM objectives Olympus offers special focus shift corrected objectives for the use with the confocal FluoView systems. Those objectives are corrected from 400 to 750nm and show no depth shift between e.g. blue and red fluorescence, making them indispensable for e.g. co-localization analyses. |
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