| Laser light source |
Ultraviolet / Visible light
LD lasers: |
405nm(22mW), 473nm(15mW), 559nm(18mW), 635nm(12mW) |
| Modulation |
Continuously Variable by the LD direct modulation(0.1%-100%, 0.1% inclement)
Line return period - laser OFF |
| Scanning |
Scanning method |
2 galvanometer scanning mirrors |
| Scanning mode |
Pixel size: 256 × 2564 - 1024 × 1024
Scanning speed: 1.1 s/frame (for pixel size 512 × 512, High Speed scanning mode)
Focusing scanning: High frame rate scan by Y- direction interlace scanning (×1, ×2, ×4)
Dimension: XYT, XYZ, XYZT
Rotation scanning: 0-359.9° in 0.1° increments |
| Detection |
Detector module |
Fluorescence: 2 channels, Phase Contrast: 1 channel
Variable barrier filter mechanism for fluorescence channel by diffraction grating and slit |
| Detection method |
Analog integration detection by Photomultiplier |
| Pinhole |
Single motorized pinhole
Pinhole diameter: ø50-800μm automatic setting (adjustable to ×1.0, ×1.5, ×2.0, and ×2.5) |
| Field number |
18 |
| Optical zoom |
10× objectives: 1× – 6× in 0.1x increments
60× objectives: 1× – 10× in 0.1x increments |
| Automatic exposure |
Automatic setting of the laser intensity and photomultiplier sensitivity to fluorescence intensity. |
| Focus |
Z-drive |
Motorized focus
Minimum increment: 0.01μm |
| Objectives |
Exclusively designed 10× phase contrast objective / NA 0.4(equivalent to UPLSAPO 10x)
Exclusively designed 60× phase contrast water-immersion objective / NA 1.2 (equivalent to UPLSAPO 60× W)/ with motorized correction collar (•)
Exclusively designed 60× phase contrast oil-immersion objective / NA 1.35 (equivalent to UPLSAPO 60× O) (⊕)
Remote switching from software by electric revolver |
| Automatic Focus (AF) |
Automatic detection of interface between specimen and cover glass by laser reflection light detection
Automatic detection of cover glass thickness and automatic setting of motorized correction collar (•)
|
| Water supply (•) |
Automatic water supply and air cleaning mechanism for 60x Water-immersion objective |
| Oil supply (ο) |
Manual
As supporting mechanism, automatic moving of XY stage to oil supply position when switching to 60x |
| Automatic exposure |
Automatic setting of the laser intensity and photomultiplier sensitivity to fluorescence intensity. |
| XY stage |
XY driving method |
Motorized XY stage module by stepping motor
Minimum increment: 0.3μm |
| Specimen holder |
Only the dedicated specimen holder can be mounted
For three glass bottom dishes with 35mm diameter (•)For one set of cover glass chamber (8 wells type)(•)
Culture pod (for a glass bottom dish with 35mm diameter)(•). For a glass slide
For a glass bottom dish with 35mm diameter (⊕) |
| Incubator (•) |
Room environment |
Temperature 37±1°C Humidity: more than 90%
CO2 concentration: 5% (recommended), 1 – joint fitting (ø2mm) for exterior CO2 adjustor |
| Heating method |
Non-contact heating by resistive heater mounted on frame section |
| Control device |
Controller |
Dedicated controller PC/AT-compatible
OS: Windows Vista Business, 32 bit (English version), CPU: Intel Core2Duo 3.0GHz
RAM: 2GB × 2, HDD: 320GB × 2, Special PCI-Express I/F board built-in, Media: DVD-Multi drive built-in |
| LCD monitor |
24 inch LCD monitor × 1, WUXGA (1920×1200) |
| Main software feature |
Image acquisition mode |
Map image, one shot, time-lapse (XYT), Z-stack (XYZ), Z-stack time-lapse (XYZT), multi area time-lapse (Multi Area XYT), multi
area Z-stack time-lapse (Multi Area XYZT) |
| Specimen setting |
Automatic setting for fluorescence channel and laser according to Dye selected from Dye list |
| Map image acquisition |
Automatic selection of map image of 3×3 – 9x9 fields according to 10× objective lens, and manual selection of map acquisition area |
| Multi area time-lapse |
Automatic multi area time-lapse by motorized XY stage
Setting for each registered point: Image size, scanning speed, cross talk reduction, pinhole diameter,
rotation angle, galvano zoom, acquisition channel, laser power, PMT sensitivity, Z condition
Maximum resister number: 10 items per one container
Maximum interval time: one hour
Maximum acquisition number of times: 3000 times per one point |
| Image acquisition area |
Area appointment: clipping square area in all area (minimum area: 96x96images) |
| Image display |
Display by channel, overlapping display, image in progress review |
| Cross talk reduction |
Line sequential action (2 channel), or frame sequential action (3 channel and 4 channel) |
| Acquisition image file type |
OLYMPUS image format (OIF) |
Image file type available
for viewing |
OLYMPUS image format (OIF, OIB), Multi-TIFF format (8/16 bit grey scale, index color, 24/32/48 bit color), JPEG, BMP, TIFF |
| Image editing |
LUT: pseudo color setting, contrast adjustment, Comment: inputting graphic, text, scale etc., image extraction, combination |
| 3D image construction |
3D display: AlphaBrend method, Maximum intensity projection method 3D animation display, free orientation of cross section display |
| Image processing |
Various types of image filter: Median, Enhanced Edge, etc.
Calculations: inter-image, arithmetic and logical operation |
| Image analysis |
Area and perimeter measurement, time-lapse measurement, colocalization analysis |
| Room environment |
Temperature: |
18°-28°C |
| Humidity |
30-80% (non condensing) |
self-contained, independent and mobile
