Fluorescent probes Many fluorescent dyes or fluorochromes can specifically interact with certain target molecules, like most nucleic acid stains (DAPI, ...
Single molecule fluorescence detection with TIRFM Commencing as a challenging problem in physics with the first detection of a single fluorescent molecule in condensed phase at temperatures of liquid helium single molecule fluorescence detection has ...
Spectral unmixing A major problem in live cell imaging arises from the use of different fluorochromes with overlapping spectra within one sample, impairing a ...
Time Lapse Imaging (in Life Science) Dynamic processes such as cell growth, metabolic transport and signal transduction are monitored routinely nowadays. The duration of such processes may vary from ...
Total internal reflection (=TIR) TIR is an optical phenomenon. If light is travelling through a medium with a high refractive index and strikes the interface of an optical medium with a lower refractive index at an angle greater than the ...
Total Internal Reflection Fluorescence Microscopy Total internal reflection (=TIR) is an optical phenomenon. If light is travelling through a medium with a high refractive index and strikes the interface of an optical medium with a lower refractive index at ...