Specifications for TIRFM

Ultra-sensitive fluorescence
    microscopy with unsurpassed
    Z-resolution at the cell surface
Simple adjustment of evanescent
    field illumination
Simultaneous widefield illumination

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Key FeaturesSpecificationsApplications

TIRF Illuminators, Lasers and Objectives


TIRF Illuminators, Lasers and Objectives

Name  Description Article Code
  Illuminator for Laser based TIRF Microscopy  
IX2-RFAEVA-2 Total internal reflection fluorescence illuminator for IX2 and IX frames. Dual light illumination system, both with field stop and ND slider. Laser beam angle adjustable, CE approved, class 3B laser certification. TIRF observation through eye impossible due to the laser safety regulations. Installation by service necessary. N1169882
IX2-RFACEVA For TIRFM with white light in combination with IX2-ARCEVA , also recommended for IX2-RFAEVA-2, high precision fluorescence cube cassette with six positions and manual shutter. Three precision fluorescence cubes (empty) included N1207800
     
  Laser for TIRF Microscopy  
FVL-ARIM-LASIPS Argon laser head 488nm/10mW and power supply E0438826
FVL-FDS-4XM-2 Fiber delivery system including 4 axis laser adjustment manipulator 038273
FVL-RHENE-LAS He-Ne laser head 633nm/10mW, FVL-HENE-PS required 038597
FVL-GHENE-LAS He-Ne laser head 543nm/1mW, FVL-HENE-PS required 038598
FVL-HENE-PS Laser power supply 038599
     
  Objectives for TIRF Microscopy  
PLAPON60xO/TIRFM-SP Plan Apochromat objective with 60x magnification. Especially suitable for TIRF applications. With working distance of 0.1 mm, numerical aperture of 1.45 and variable temperature/coverslip correction via correction collar: 0.13 to 0.19 mm at 20°C (room temperature), and 0.17mm from 23° to 37°C N9010775
PLAPO100xO/TIRFM-SP Plan Apochromat oil immersion objective with 100x magnification. With working distance of 0.1 mm, numerical aperture of 1.45 and cover correction of 0.17 mm. Particularly suitable for total internal reflection fluorescence microscopy (TIRFM). N9008507
APO100XO/HR Apochromatic oil immersion objective withh 100x magnification, working distance of 0.1mm, numerical aperture of 1.65 and cover correction of 0.15mm (special cover glass 9U-990). For total internal reflection fluorescence microscopy. 037683
UAPO150xO/TIRFM-SP Universal apochromat oil immersion objective with 150x magnification, working distance of 0.1 mm,  numerical aperture of 1.45, and variable temperature/coverslip correction via correction collar: 0.13-0.21 mm at 23°C (room temperature),  and 0.17 mm at 37°C. Particularly suitable for single molecule detection and total internal reflection microscopy (TIRFM). N9011689
9U-990 Special coverslip for APO100xO/HR 035055
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