| Hardware |
Olympus microscopes BX50 -BX61, BX61WI, IX50-IX81 |
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CCD, EMCCD cameras, various models |
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Imaging Computer (latest generation PC) |
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| Illumination system |
MT10 |
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Short arc burners, |
150 W Xenon or Mercury-Xenon |
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8 Filter positions |
diameter 25 mm |
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Filter switch |
min. 85 ms (neighbouring positions) |
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Attenuation |
7 levels, 4% - 100% |
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Attenuation switch |
<85 ms |
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Shutter, on/off time |
5 ms |
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Operation |
sequentially |
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| Hardware control |
Control board with additional CPU, independent from imaging PC |
System coordinator |
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Temporal resolution |
10 ms (camera 1 ms) |
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Timing precision |
ca. 15 ms |
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Camera control |
trigger (level trigger if accepted by camera) |
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Peripheral device control via TTL pulses |
optional: 3 BNC connectors |
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Multi-task acquisition with hardware switch (z-position, exciter filter etc.), 50 ms exposure |
3-4 full frames/sec with sequential hardware switch |
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| Experiment set-up and control |
Graphical interface Experiment Manager |
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Drag-and-drop alignment of command icons |
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7-D acquisition (xyz, excitation and emission colour, time, stage position) |
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Loop-in-loop capability (repetition of complex command groups) |
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Experiments with varying acquisition speed and camera exposure |
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Autofocus, repeatedly during experiment |
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Live image display and online analysis |
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User interaction: pause, resume, set marker |
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| Imaging software |
cell^M |
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Structured database for multi-dimensional data handling and storage |
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Image types: (n x 16) bit, 8 - 24 bit export and import |
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Image processing: filters, extended focal imaging, shading and background correction, arithmetic… |
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Measurements and analyses: number, length, distance, area, circumference, angle, grey value, histograms, line profiles, tables, statistics, diagrams… |
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Fluorescence analyses: intensity kinetics, ratioing, Δ F/F |
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Spectral unmixing for optimised colour resolution |
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Deblurring, SliceViewer, VoxelViewer |
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Macros and automated functions: imaging C module, macro recorder |
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| Options |
Piezo-electric objective drive (all microscopes) or nosepiece drive (IX51, IX71, IX81 only) |
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TIRFM lasers and illumination combiners, multi-line |
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FRET, hardware and analysis (different algorithms) |
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Deconvolution for resolution enhancement |
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3-D rendering for three-dimensional image generation |
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Particle detection and tracking |
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| Upgradeability |
To cell^R |
Multi-fluorescence 3-D time-lapse
