Specifications for cell^tool TIRFM |
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 Cell surface observation without
out-of-focus blur
Fully integrated into cell^M and ^R imaging stations
Combination of up to three lasers
plus a MT10/20 fluorescence
illumination system
Optimised beam alignment on
individual laser ports
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Multi-port Illuminator
| Multi-port Illuminator |
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Modular design for the combination of up to three lasers plusa MT10/MT20 fluorescence illumination system |
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Individual ports for each laser fibre guarantee optimised beam alignment |
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Field stop for each laser port to reduce the field of illumination |
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Fast shutters (1ms) allow fast switch between all lasers and MT10/MT20 |
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Easy experiment set-up by integration into the Experiment Manager of cell^M and cell^R |
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Including all safety features required by laser safety regulations |
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Class 1 laser product (normal operation), Class 3B (maintenance mode), IEC60825:1 |
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Available for IX2 microscopes |
TIRFM Objectives
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Objectives for TIRF Microscopy |
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| PLAPON60xO/TIRFM-SP |
Plan Apochromat objective with 60x magnification. Especially suitable for TIRF applications. With working distance of 0.1 mm, numerical aperture of 1.45 and variable temperature/coverslip correction via correction collar: 0.13 to 0.19 mm at 20°C (room temperature), and 0.17mm from 23° to 37°C. |
N9010775 |
| PLAPO100xO/TIRFM-SP |
Plan Apochromat oil immersion objective with 100x magnification. With working distance of 0.1 mm, numerical aperture of 1.45 and cover correction of 0.17 mm. Particularly suitable for total internal reflection fluorescence microscopy (TIRFM). |
N9008507 |
| APO100XO/HR |
Apochromatic oil immersion objective withh 100x magnification, working distance of 0.1mm, numerical aperture of 1.65 and cover correction of 0.15mm (special cover glass 9U-990). For total internal reflection fluorescence microscopy. |
037683 |
| UAPO150xO/TIRFM-SP |
Universal apochromat oil immersion objective with 150x magnification, working distance of 0.1 mm, numerical aperture of 1.45, and variable temperature/coverslip correction via correction collar: 0.13-0.21 mm at 23°C (room temperature), and 0.17 mm at 37°C. Particularly suitable for single molecule detection and total internal reflection microscopy (TIRFM). |
N9011689 |
| 9U-990 |
Special coverslip for APO100xO/HR |
035055 |
TIRFM Laser
| TIRFM Laser |
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Diode or diode-pumped solid state lasers |
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Five wavelengths available: 405 nm (50 mW), 488 nm (30 mW), 532 nm (20 or 50 mW), 561 nm (25 mW) and 635 nm (25 mW) |
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Laser coupling via polarisation-maintaining single mode fibre |
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20 levels of attenuation between about 0.2% and 100% |
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Fast shutter with 1 ms switching time |
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Expected lifetime up to 10,000 h |
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Low operating noise |
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Compact housing |
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Control via hand switch, and / or external TTL trigger |
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Complying laser safety regulations |
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Class 3B laser product (IEC60825:1) |
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