Applications for F-View II
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 Resolution: 1376 x 1032 pixels
Binning: 2x, 4x, 8x
Dynamic range: 12 bits
Exposure times: 100 µs – 160 sec.
Frame rates: > 22 fps @ 2x binning
(live)
Cooling: Peltier cooled
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Becke line |
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The Becke line appears as a band of light along the boundary between two media with different... More
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Binning |
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Binning, also called on-chip integration, is the combination of the charges of neighboring CCD pixels during readout.... More
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Bit Depth of Camera Data |
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The bit depth refers to the digitization of the analogue light signal by the analogue-to-digital (A/D) converter... More
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Blooming |
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If a CCD is overexposed, that is, if the illumination exceeds the full-well capacity of the photodiodes... More
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Cameleon |
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The protein Calmodulin is a ubiquitous intracellular calcium sensor that responds to changes in calcium concentration. Each... More
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CCD |
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The acronym CCD stands for "charge-coupled device". CCDs are used as electronic light sensors: Photons hitting the... More
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CCD Chip Size |
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The CCD chip size is often given in an inch measure: 1/2" chip, 2/3" chip, etc. Counter... More
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CCD Chip Types |
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Back-thinned, back-illuminated CCD chips
This is a rather expensive and delicate type of chips for high-end scientific-grade... More
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Chimeric proteins |
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The construction of chimeric proteins is an elegant approach for FRET analysis in living cells (e.g.... More
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Data Readout Rate |
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Usually the rate limiting step is analogue-to-digital signal conversion. The conversion rate ranges somewhere between 1 and... More
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Digital images |
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Digital image files are based on tables that list the intensities, or "counts", of all pixels. In... More
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Dynamic Range |
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The dynamic range of a CCD camera (DR) is the maximum signal (which is directly related to... More
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Epodye |
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Epodye is a fluorescent dye (Struers), which can be added to a cold mounting
resin. When the... More
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FireWire |
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The IEEE1394, also called FireWire, is a modern plug-and-play interface between PC and camera (or other peripherals,... More
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Fluorescence |
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The term fluorescence is derived from the mineral fluorspar (calcium fluoride). Fluorescence belongs to the photoluminescence group... More
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Fluorochromes |
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Fluorochromes, unlike classical tissue stains, re-radiate the absorbed light, however, with longer wavelength (red-shifted). Each fluorochrome has... More
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Full-Well Capacity |
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This is the maximum amount of charges (electrons) the photodiodes of a CCD chip can accommodate. It... More
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Ion imaging |
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The fluorescence behaviour of several dyes is dependent on the concentration of certain ions such as calcium... More
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Pixels |
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Digital images are made up of many thousands or even millions of tiny squares (or rectangles) called... More
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Shading |
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Gradual noise and sensitivity variations across the CCD chip and the corresponding inherent intensity gradients in the... More
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Slow-Scan Cameras |
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CCD cameras are often termed slow-scan cameras because their standard frame rate is usually less than that... More
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Spectral unmixing |
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A major problem in live cell imaging arises from the use of different fluorochromes with overlapping spectra... More
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Well depth |
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The well depth is the maximum amount of charges (electrons) the photodiodes of a CCD chip can... More
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F-View II related Application Report |
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